Application

This Anti-Scribble Antibody is validated for use in WB for the detection of Scribble.

Suggested dilutions:
Western blot: 0.2 µg (1:2000)

Research Sub CategoryTumor MarkersCytoskeleton

General description

Scribble is a tumor suppressor marker first discovered in Drosophila that has been implicated in the control of cell proliferation, polarity, invasion, and metastasis (Humbert, et al, 2003). Mammalian Scribble expresses basolateral staining in epithelial tissue and highly polarized expression in T lymphocytes (Dow, et al, 2003) (Ludford-Menting, et al, 2005), and plays an essential role in the regulation of the polarity specifically involved in directed epithelial migration. Altered expression of human Scribble is associated with invasive epithelial cancers, however, its role in tumour development remains unclear (Dow, et al, 2007).

Immunogen

Epitope: PDZ2 domain of Scribble

GST-tagged fusion protein corresponding to the PDZ2 domain of Scribble

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified in PBS containing with 0.05% NaN3.

Format: Purified

Quality

Routinely evaluated by Western blot on HeLa cell lysates.

Specificity

Predicted to cross react with mouse based on sequence homology. Reactivity with other species has not been tested.

Storage and Stability

Maintain at 2-8°C for up to 1 year after date of receipt.

Target description

220 kDa